Quick Overview for LDHB Protein (AA 1-334) (ABIN6387839)
抗原
See all LDHB 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-334
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序列
- MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
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Lactate Dehydrogenase B (LDHB) (AA 1-334) protein (GST tag)
LDHB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase B (LDHB) (AA 1-334) (Active) protein (His tag)LDHB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, EAA Active
Lactate Dehydrogenase B (LDHB) (AA 2-334), (full length) protein (His tag)LDHB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl ( pH 8.0) containing 10 % glycerol, 1 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- LDHB (Lactate Dehydrogenase B (LDHB))
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别名
- Lactate Dehydrogenase B/LDHB
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背景
- LDHB, also known as L-lactate dehydrogenase B chain isoform LDHB, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate. The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 36.6 kDa (334aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_002291
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UniProt
- P07195
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途径
- Warburg Effect
抗原
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