LDHB Protein (AA 1-334) (His tag)
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Quick Overview for LDHB Protein (AA 1-334) (His tag) (ABIN5854963)
抗原
See all LDHB 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-334
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标记
- This LDHB protein is labelled with His tag.
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序列
- MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 150unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.
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Lactate Dehydrogenase B (LDHB) (AA 1-334) protein (GST tag)
LDHB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase B (LDHB) (AA 1-334) protein (His-IF2DI Tag)LDHB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Lactate Dehydrogenase B (LDHB) (AA 1-334) (Active) proteinLDHB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, EAA Active
Lactate Dehydrogenase B (LDHB) (AA 2-334), (full length) protein (His tag)LDHB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- LDHB (Lactate Dehydrogenase B (LDHB))
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别名
- Lactate Dehydrogenase B/LDHB
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背景
- Ldhb, also known as L-lactate dehydrogenase B chain isoform Ldhb, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant mouse Ldhb protein, fused to His-tagging at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 39 kDa (357aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_032518
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途径
- Warburg Effect
抗原
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