CSTA Protein (AA 1-98) (His tag)
Quick Overview for CSTA Protein (AA 1-98) (His tag) (ABIN6387718)
抗原
See all CSTA 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-98
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标记
- This CSTA protein is labelled with His tag.
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序列
- MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF
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Biological Activity Comment
- The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (His tag)
custom-made CSTA 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cystatin A (Stefin A) (CSTA) (AA 1-97) protein (His tag)custom-made CSTA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)CSTA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- CSTA (Cystatin A (Stefin A) (CSTA))
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别名
- CSTA
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背景
- CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies Recombinant human CSTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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分子量
- 13.1 kDa (118aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_005204
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UniProt
- P01040
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途径
- Response to Water Deprivation
抗原
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