MAP4K3 Protein (AA 1-321) (His tag)
Quick Overview for MAP4K3 Protein (AA 1-321) (His tag) (ABIN6387691)
抗原
See all MAP4K3 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-321
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标记
- This MAP4K3 protein is labelled with His tag.
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序列
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MTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL -
Biological Activity Comment
- Specific activity is > 70unit/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-894) protein (His tag)
custom-made MAP4K3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-894) protein (His tag)custom-made MAP4K3 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-321) protein (His tag)MAP4K3 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) protein (Myc-DYKDDDDK Tag)MAP4K3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Bioactivity Validated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
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别名
- glk
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背景
- Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 37.1 kDa (344aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_416889
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UniProt
- P0A6V8
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途径
- MAPK Pathway
抗原
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