GLO1 Protein (AA 1-184) (His tag)
Quick Overview for GLO1 Protein (AA 1-184) (His tag) (ABIN6387621)
抗原
See all GLO1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 1-184
-
标记
- This GLO1 protein is labelled with His tag.
-
序列
- ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH
-
内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
-
Biological Activity Comment
- Specific activity: > 400unit/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.
-
-
Glyoxalase I (GLO1) (AA 1-184) protein (GST tag)
GLO1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyoxalase I (GLO1) (AA 2-184) (Active) protein (His tag)GLO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Glyoxalase I (GLO1) (AA 1-184) proteinGLO1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Glyoxalase I (GLO1) (AA 1-184) (Active) protein (His tag)GLO1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, EAA Active
Glyoxalase I (GLO1) protein (His tag)GLO1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Glyoxalase I (GLO1) protein (Myc-DYKDDDDK Tag)GLO1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Bioactivity Validated
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
-
- GLO1 (Glyoxalase I (GLO1))
-
别名
- Glyoxalase I
-
背景
- Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
-
分子量
- 20.7 kDa (184aa) confirmed by MALDI-TOF
-
NCBI登录号
- NP_006699
-
UniProt
- Q04760
抗原
-
