VEGF 165 (AA 27-191) protein (His tag)
-
- 抗原
- VEGF 165
- 蛋白类型
- Recombinant
- 产品特性
- AA 27-191
-
宿主
- 人
-
资源
- Baculovirus infected Insect Cells
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 序列
-
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
- 纯度
- > 85% by SDS-PAGE
- 内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate buffered saline ( pH 7.4), 30 % glycerol, 1 mM DTT, 0.1 mM PMSF
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- VEGF 165
- 背景
- Vascular endothelial growth factor A, also known as VEGF, is growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. This protein binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Recombinant human VEGF165 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
- 分子量
- 19.9 kDa (171aa)
- NCBI登录号
- NP_001165097
- UniProt
- P15692
-