L-Lactate Dehydrogenase (LLDD) (AA 1-396) protein (His tag)
LLDD
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90% by SDS-PAGE
SDS
1 image
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- 抗原
- L-Lactate Dehydrogenase (LLDD)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-396
- 宿主
- E. coli
- 资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSHMIISAA SDYRAAAQRI LPPFLFHYMD GGAYSEYTLR RNVEDLSEVA LRQRILKNMS DLSLETTLFN EKLSMPVALA PVGLCGMYAR RGEVQAAKAA DAHGIPFTLS TVSVCPIEEV APAIKRPMWF QLYVLRDRGF MRNALERAKA AGCSTLVFTV DMPTPGARYR DAHSGMSGPN AAMRRYLQAV THPQWAWDVG LNGRPHDLGN ISAYLGKPTG LEDYIGWLGN NFDPSISWKD LEWIRDFWDG PMVIKGILDP EDARDAVRFG ADGIVVSNHG GRQLDGVLSS ARALPAIADA VKGDIAILAD SGIRNGLDVV RMIALGADTV LLGRAFLYAL ATAGQAGVAN LLNLIEKEMK VAMTLTGAKS ISEITQDSLV QGLGKELPAA LAPMAKGNAA
- 纯化方法
- purified by using conventional chromatography.
- 纯度
- > 90% by SDS-PAGE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 MM DTT
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- 抗原
- L-Lactate Dehydrogenase (LLDD)
- 别名
- L-lactate dehydrogenase/LLDD
- 背景
- LldD, also known as L-lactate dehydrogenase, is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). Recombinant E. coli lldD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
- 分子量
- 45.3 kDa (420aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_418062
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