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Osteopontin Protein (His tag,GST tag)

This Recombinant Osteopontin protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN6381875
发货至: 中国
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Quick Overview for Osteopontin Protein (His tag,GST tag) (ABIN6381875)

抗原

See all Osteopontin (SPP1) 蛋白
Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

蛋白类型

Recombinant

宿主

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Cow

资源

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大肠杆菌(E. Coli)

应用范围

Western Blotting (WB), SDS-PAGE (SDS)

纯度

> 80 %
  • 标记

    This Osteopontin protein is labelled with His tag,GST tag.

    原理

    Cow Osteopontin (SPP1) Protein

    序列

    LPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ KQTFLAPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDDNSQDVNS NDSDDAETTD DPDHSDESHH SDESDEVDFP TDIPTIAVFT PFIPTESAND GRGDSVAYGL KSRSKKFRRS NVQSPDATEE DFTSHIESEE MHDAPKKTSQ LTDHSKETNS SELSKELTPK AKDKNKHSNL IESQENSKLS QEFHSLEDKL DLDHKSEEDK HLKIRISHEL DSASSEVN

    Biological Activity Comment

    Not tested
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Please note that the majority of this manufacturers proteins are partial length rather than full length.
    We recommend customers to inquire.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

    缓冲液

    Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.

    储存条件

    4 °C,-80 °C

    储存方法

    Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

    别名

    Osteopontin

    分子量

    Calculated MW: 59.2 kDa
    Observed MW: 71 kDa

    Possible reasons why the actual band size differs from the predicted band size:
    1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
    2. Rela

    UniProt

    P31096

    途径

    Regulation of Cell Size
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