Flagellin Protein (FliC) (Mutant) (His tag)
Quick Overview for Flagellin Protein (FliC) (Mutant) (His tag) (ABIN6253486)
抗原
See all Flagellin (FliC) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- Mutant
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标记
- This Flagellin protein is labelled with His tag.
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原理
- Flagellin (TLR5 Mutant) (rec.)
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特异性
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
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交叉反应
- 人, 小鼠
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产品特性
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
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内毒素水平
- <0.01EU/μg purified protein (LAL test).
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Biological Activity Comment
- Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
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Flagellin (FliC) (AA 2-498) protein (His tag)
FliC 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Flagellin (FliC) proteinFliC 宿主: Borrelia burgdorferi 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, WB, SDS, CP
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- 0.1 mg/mL
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缓冲液
- Contains PBS.
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注意事项
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
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有效期
- 6 months
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- Flagellin (FliC)
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别名
- Flagellin
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背景
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FliC (TLR5 Mutant)
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.
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途径
- Inflammasome
抗原
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