MBL2 Protein (AA 130-248) (His tag,GST tag)
This MBL2 protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for MBL2 Protein (AA 130-248) (His tag,GST tag) (ABIN6239795)
抗原
See all MBL2 蛋白
MBL2
(Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Activity Assay (AcA), Cell Culture (CC)
纯度
> 97 %
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产品特性
- AA 130-248
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标记
- This MBL2 protein is labelled with His tag,GST tag.
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产品特性
- Tag location: N-terminal His and GST Tag
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Biological Activity Comment
- MBL2 (Mannose-binding protein C) is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different microorganisms, therefore results in activation of the lectin pathway of the complement system. It has been proven that MASP-2 (Mannan-binding lectin serine protease 2) forms complexes with the pattern recognition molecules MBL2, triggers the activation of the complement system. Thus, a functional binding ELISA assay was constructed to detect the association of rhMBL2 with MASP2. Briefly, rhMBL2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MASP2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MBL2 mAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MBL2 with MASP2 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of MBL2 with MASP2.
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Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) protein
MBL2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) protein (His tag)MBL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
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应用备注
- Isoelectric Point: 5.8
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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储存液
- Dithiothreitol (DTT), Other preservative, ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
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别名
- Mannose Binding Lectin (MBL)
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背景
- Alternative Names: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
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分子量
- 43kDa
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UniProt
- P11226
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途径
- Complement System, Positive Regulation of Immune Effector Process
抗原
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