ACP5 Protein (AA 22-325) (His tag)
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- 抗原 See all ACP5 蛋白
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 22-325
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This ACP5 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- 序列
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ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARP
- 纯度
- > 95% by SDS-PAGE
- 内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 5,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.
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Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 22-325) (Active) protein
ACP5 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, EAA Active
Acid Phosphatase 5, Tartrate Resistant (ACP5) (Active) protein (His tag)ACP5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Acid Phosphatase 5, Tartrate Resistant (ACP5) (AA 1-325) protein (GST tag)ACP5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- 抗原
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
- 别名
- TRACP/PAP/ACP5 (ACP5 产品)
- 背景
- ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 35.1 kDa (310aa)
- NCBI登录号
- NP_001104505
- UniProt
- P13686
- 途径
- Transition Metal Ion Homeostasis
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