Dynamitin Protein (AA 1-406) (His tag)
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Quick Overview for Dynamitin Protein (AA 1-406) (His tag) (ABIN5853454)
抗原
See all Dynamitin (DCTN2) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-406
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标记
- This Dynamitin protein is labelled with His tag.
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序列
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MADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAFA QELEELTSTS VEHIIVNPNA AYDKFKDKRV GTKGLDFSDR IGKTKRTGYE SGEYEMLGEG LGVKETPQQK YQRLLHEVQE LTTEVEKIKT TVKESATEEK LTPVLLAKQL AALKQQLVAS HLEKLLGPDA AINLTDPDGA LAKRLLLQLE ATKNSKGGSG GKTTGTPPDS SLVTYELHSR PEQDKFSQAA KVAELEKRLT ELETAVRCDQ DAQNPLSAGL QGACLMETVE LLQAKVSALD LAVLDQVEAR LQSVLGKVNE IAKHKASVED ADTQSKVHQL YETIQRWSPI ASTLPELVQR LVTIKQLHEQ AMQFGQLLTH LDTTQQMIAN SLKDNTTLLT QVQTTMRENL ATVEGNFASI DERMKKLGK -
纯化方法
- > 90% by SDS-PAGE
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Dynactin 2 (p50) (DCTN2) (AA 1-406) protein (His-IF2DI Tag)
DCTN2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Dynactin 2 (p50) (DCTN2) (AA 1-401) protein (GST tag)DCTN2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dynactin 2 (p50) (DCTN2) (AA 1-403) protein (His tag)DCTN2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Dynactin 2 (p50) (DCTN2) (AA 1-401) protein (His tag)DCTN2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Dynactin 2 (p50) (DCTN2) protein (Myc-DYKDDDDK Tag)DCTN2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.15M NaCl, 1 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))
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别名
- Dynactin Subunit 2/DCTN2
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背景
- DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Recombinant human DCTN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 47.2 kDa (429aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_006391
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途径
- M Phase, Ribonucleoprotein Complex Subunit Organization
抗原
-
