RPA3 Protein (AA 1-119, partial) (GST tag)
This Recombinant RPA3 protein is expressed in 大肠杆菌（E. Coli）.
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产品编号 ABIN5713006
发货至:
中国
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Quick Overview for RPA3 Protein (AA 1-119, partial) (GST tag) (ABIN5713006)
抗原
See all RPA3 蛋白
RPA3
(Replication Protein A3, 14kDa (RPA3))
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 90 %
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产品特性
- AA 1-119, partial
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标记
- This RPA3 protein is labelled with GST tag.
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序列
- MVDMMDLPRS RINAGMLAQF IDKPVCFVGR LEKIHPTGKM FILSDGEGKN GTIELMEPLD EEISGIVEVV GRVTAKATIL CTSYVQFKED SHPFDLGLYN EAVKIIHDFP QFYPLGIVQ
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纯化方法
- SDS-PAGE
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Replication Protein A3, 14kDa (RPA3) (AA 1-121) protein (GST tag)
RPA3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Replication Protein A3, 14kDa (RPA3) (AA 1-119), (partial) protein (His tag)RPA3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % SDS, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1-2 mg/mL
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缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
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储存条件
- -80 °C,4 °C,-20 °C
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储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- RPA3 (Replication Protein A3, 14kDa (RPA3))
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别名
- RFA3
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背景
- As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage . In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response . It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair . Plays also a role in base excision repair (BER), probably through interaction with UNG . Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA . As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange .
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分子量
- 40.7 kDa
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UniProt
- P35244
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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