CD14 Protein (CD14) (His tag)
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- 抗原 See all CD14 蛋白
- CD14
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- CHO Cells
- 标记
- This CD14 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Agonist (Agon)
- 序列
- SPAPPEPCEL DEESCSCNFS DPKPDWSSAF NCLGAADVEL YGGGRSLEYL LKRVDTEADL GQFTDIIKSL SLKRLTVRAA RIPSRILFGA LRVLGISGLQ ELTLENLEVT GTAPPPLLEA TGPDLNILNL RNVSWATRDA WLAELQQWLK PGLKVLSIAQ AHSLNFSCEQ VRVFPALSTL DLSDNPELGE RGLISALCPL KFPTLQVLAL RNAGMETPSG VCSALAAARV QLQGLDLSHN SLRDAAGAPS CDWPSQLNSL NLSFTGLKQV PKGLPAKLSV LDLSYNRLDR NPSPDELPQV GNLSLKGNPF LDSESHSEKF NSGVVTAGAP SSQAVALSGT LALLLGDRLF VHHHHHH
- 产品特性
- The recombinant mouseCD14 is produced from mouse CD14 transfected CHO-cells in serum free medium, has been purified by His-tag with Talon (Clontech) and controlled by SDS page. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.
- 纯化方法
- Affinity chromatography by His-Tag
- 纯度
- >95 % by SDS-PAGE
- 内毒素水平
- <1 EU/μgof recombinant mouse CD14 as determined by LAL method
- Biological Activity Comment
- Up to 5μg/ml mouse CD14 inhibit binding of FITC-LPS (0.5μg/ml) to mouse CD14+CHO transfectants, don't activate monocytes itself
- Top Product
- Discover our top product CD14 蛋白
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 20-344) protein (His tag)CD14 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD14 (CD14) protein (His tag,Fc Tag)CD14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL
- 浓度
- 0.25 mg/mL
- 缓冲液
- sterile-filtered phosphate balanced salt solution, pH 7.5
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C,-80 °C
- 储存方法
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Storage: -20°C or -70°C
Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months.The lyophilized product thought stable at -70oC more than 1 year, at refrigerator about 10 months at room temperature up to 4 weeks, and at 37oC 1 week. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided.
- 有效期
- 12 months
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- 抗原
- CD14
- 别名
- CD14 (CD14 产品)
- 别名
- CD14 molecule Protein, CD14 antigen Protein, CD14 Protein, Cd14 Protein
- 背景
- Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macro-phages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.
- 分子量
- ~50kDa
- 基因ID
- 12475
- NCBI登录号
- NP_033971
- UniProt
- P10810
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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