Quick Overview for YBX1 蛋白 (ABIN5624618)
抗原
See all YBX1 蛋白蛋白类型
宿主
资源
应用范围
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Supplier Product No.
- 000-001-c16
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Supplier
- Rockland
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原理
- DbpB Control Protein
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纯化方法
- DbpB is a fusion protein synthesized with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified DbpB and was estimated to be greater than 90% pure.
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过滤
- Sterile filtered
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Y Box Binding Protein 1 (YBX1) (AA 1-324) protein (GST tag)
YBX1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Y Box Binding Protein 1 (YBX1) (AA 1-324) protein (His tag)YBX1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Y Box Binding Protein 1 (YBX1) (AA 51-139) protein (GST tag)YBX1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Y Box Binding Protein 1 (YBX1) (AA 2-322) protein (His tag)YBX1 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % SDS
Y Box Binding Protein 1 (YBX1) protein (Myc-DYKDDDDK Tag)YBX1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: DbpB protein has been tested by SDS-PAGE and Western blotting and is suitable as a control in other immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.3 kDa for DbpB-MBP, (17.9 kDa for DbpB and 42.4 kDa for MBP) in size corresponding to DbpB by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: Lateral Flow Assay: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
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有效期
- 6 months
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- YBX1 (Y Box Binding Protein 1 (YBX1))
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别名
- DbpB
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背景
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Synonyms: Decorin-binding protein B, Borrelia burgdorferi DbpB, control protein, DbpB Control Protein
Background: Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B. burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
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基因ID
- 1194341
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NCBI登录号
- WP_010890381
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UniProt
- P0CL68
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途径
- Regulation of Muscle Cell Differentiation
抗原
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