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Flagellin Protein (FliC)

This Recombinant Flagellin protein is expressed in 大肠杆菌(E. Coli).
Rockland
产品编号 ABIN5624606
Supplier Product No.: 000-001-c14
发货至: 中国

Quick Overview for Flagellin Protein (FliC) (ABIN5624606)

抗原

See all Flagellin (FliC) 蛋白
Flagellin (FliC)

蛋白类型

Recombinant

宿主

  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Borrelia burgdorferi

资源

  • 7
  • 5
  • 2
大肠杆菌(E. Coli)

应用范围

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Control Peptide (CP)
  • Supplier Product No.

    000-001-c14

    Supplier

    Rockland

    原理

    Flagellin Control Protein

    纯化方法

    Flagellin is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Flagellin and was estimated to be greater than 90% pure.

    过滤

    Sterile filtered
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  • 应用备注

    Application Note: Flagellin is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 76.3 kDa Flagellin-MBP, (33.9 kDa for Flagellin and 42.4 kDa for MBP) in size corresponding to Flagellin by Western blotting in the appropriate cell lysate or extract. Flagellin Protein was tested in SDS-page and western blot.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    Other: Lateral Flow Assay: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

    有效期

    6 months
  • 抗原

    Flagellin (FliC)

    别名

    Flagellin

    背景

    Synonyms: 41 kDa antigen, Borrelia burgdorferi p41, fla, Flagellar filament 41 kDa core protein, Bacterial flagellin, control protein

    Background: Flagellin is a protein found in the hollow cylinder forming the filament in bacterial flagellum. Its structure is helical, which is important for its function. Studies comparing a flagellate Borrelia to flagellated indicate that the flagella have a role in the invasion of human tissue. The N- and C-termini of flagellin form the inner core of the flagellar filament, and the central portion of the protein makes up the outer surface. While the terminus of the protein is quite similar between all bacterial flagellins, the central portion is variable. The flagellin genes are highly conserved among the different Borrelia species. Mammals often have acquired immune responses (T-cell and antibody responses) to flagellated bacterium. Some bacteria are able to switch between multiple flagellin genes in order to evade this response. Borrelia burgdorferi, the spirochete that is associated with Lyme Disease, may use this tactic when challenging mammals with infection. Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids, in low copy number. This suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host, i.e., changes in environment as the spirochete migrates from the tick's midgut to its salivary glands to the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

    基因ID

    7106737

    NCBI登录号

    WP_002661938

    UniProt

    P11089

    途径

    Inflammasome
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