TIGIT Protein (AA 22-141) (Fc Tag,AVI tag,Biotin)
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Quick Overview for TIGIT Protein (AA 22-141) (Fc Tag,AVI tag,Biotin) (ABIN4949040)
抗原
See all TIGIT 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 22-141
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标记
- This TIGIT protein is labelled with Fc Tag,AVI tag,Biotin.
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品牌
- MABSol® - PrecisionAvi
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序列
- AA 22-141
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特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
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产品特性
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This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 41.5 kDa. As a result of glycosylation, the protein migrates as 47 kDa under reducing (R) condition, and 75-116 kDa under non-reducing (NR) condition (SDS-PAGE).
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization. -
内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Biotin,His-Avi Tag)
TIGIT 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) protein (Fc Tag)TIGIT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (His tag)TIGIT 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 26-143) (Active) protein (His tag)TIGIT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) protein (mFc Tag)TIGIT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) protein (His tag)TIGIT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 89-208) (Active) protein (His tag)TIGIT 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Func Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag)TIGIT 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) (AA 22-141) (Active) protein (Fc Tag,AVI tag,Biotin)TIGIT 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT) protein (His tag)TIGIT 宿主: Cynomolgus 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
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说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Tris with Glycine, Arginine and NaCl, pH 7.5
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注意事项
- Please avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
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别名
- TIGIT
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背景
- T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
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分子量
- 41.3 kDa
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NCBI登录号
- NP_776160
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途径
- Cancer Immune Checkpoints
抗原
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