LacI (AA 1-360) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for LacI (AA 1-360) protein (His tag) (ABIN7312770)
抗原
LacI
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
ELISA, Mass Spectrometry (MS), SDS-PAGE (SDS), Western Blotting (WB)
纯度
Greater than 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1-360
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标记
- His tag
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原理
- Recombinant E. coli LACI Protein is produced by our E. coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.
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序列
- MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as a 0.2 μM filtered solution of 20 mM Tris, 300 mM NaCl, 5 mM DTT, pH 8.0.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- LacI
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基因ID
- 945007
抗原
-
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