TIGAR Protein (AA 1-269) (Strep Tag)
Quick Overview for TIGAR Protein (AA 1-269) (Strep Tag) (ABIN3136329)
抗原
See all TIGAR 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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产品特性
- AA 1-269
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标记
- This TIGAR protein is labelled with Strep Tag.
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序列
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MPRFALTVIR HGETRLNKEK IIQGQGVDAP LSETGFRQAA AAGQFLSNVQ FTHAFSSDLT RTKQTIHGIL EKSRFCKDMA VKYDSRLRER MYGVAEGKPL SELRAMAKAA GEECPMFTPP GGETVEQVKM RGKDFFDFIC QLILGKAGQR ESVLPGAPGS GLESSLAEVF PVGKHGSLGA NPKGGTLGLA ASILVVSHGA YMRSLFGYFL SDLRCSLPGA RDKLELSSIT PNTGISVFII DCEEARQPSI QCVCMNLQEH LNGVTEKQH
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. -
产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
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纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin MousePrice 8,182.91 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-269) protein (His tag)
custom-made TIGAR 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (His tag)custom-made TIGAR 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 2-270) protein (His tag)TIGAR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (GST tag)TIGAR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. -
注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
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别名
- Tigar
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背景
- Fructose-2,6-bisphosphatase TIGAR (EC 3.1.3.46) (TP53-induced glycolysis and apoptosis regulator) (TP53-induced glycolysis regulatory phosphatase),FUNCTION: Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (By similarity). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:23726973). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:23726973). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (By similarity). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3 (PubMed:22044588). Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (PubMed:22044588). Plays a role in adult intestinal regeneration, contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (PubMed:24872551). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner. Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (By similarity). Involved in intestinal tumor progression (PubMed:23726973). {ECO:0000250|UniProtKB:Q9NQ88, ECO:0000269|PubMed:22044588, ECO:0000269|PubMed:23726973, ECO:0000269|PubMed:24872551}.
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分子量
- 29.2 kDa
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UniProt
- Q8BZA9
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途径
- Warburg Effect
抗原
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