GTF2H1 Protein (AA 1-547) (Strep Tag)
Quick Overview for GTF2H1 Protein (AA 1-547) (Strep Tag) (ABIN3129537)
抗原
See all GTF2H1 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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产品特性
- AA 1-547
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标记
- This GTF2H1 protein is labelled with Strep Tag.
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序列
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MATSSEEVLL IVKKVRQKKQ DGALYLMAER IAWAPEGKDR FTISHMYADI KCQKISPEGK AKIQLQLVLH AGDTTNFHFS NESTAVKERD AVKDLLQQLL PKFKRKANKE LEEKNRMLQE DPVLFQLYKD LVVSQVISAE EFWANRLNVN ATDSSTSSHK QDVGISAAFL ADVRPQTDGC NGLRYNLTSD IIESIFRTYP AVKMKYAETV PHNMTEKEFW TRFFQSHYFH RDRLNTGSKD LFAECAKIDE KGLKTMVSLG VKNPMLDLTS LEDKPLDEGY SISSVPSTSN SKSIKENSNA AIIKRFNHHS AMVLAAGLRK QQAQNGQNGE PSSVDGNSGD TDCFQPAVKR AKLQESIEYE DLGNNNSVKT IALNLKKSDR YYHGPTPIQS LQYATSQDII NSFQSIRQEM EAYTPKLTQV LSSSAASSTI TALSPGGALM QGGTQQAVNQ MVPNDIQSEL KHLYVAVGEL LRHFWSCFPV NTPFLEEKVV KMKSNLERFQ VTKLCPFQEK IRRQYLSTNL VSHIEEMLQT AYNKLHTWQS RRLMKKT
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. -
产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
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纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin MousePrice 6,177.32 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin MousePrice 19,196.78 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1) (AA 1-548) protein (His tag)
custom-made GTF2H1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1) (AA 1-547) protein (His tag)custom-made GTF2H1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1) (AA 1-548) protein (GST tag)GTF2H1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GTF2H1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. -
注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- GTF2H1 (General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1))
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别名
- Gtf2h1
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背景
- General transcription factor IIH subunit 1 (Basic transcription factor 2 62 kDa subunit) (BTF2 p62) (General transcription factor IIH polypeptide 1) (TFIIH basal transcription factor complex p62 subunit),FUNCTION: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. {ECO:0000250|UniProtKB:P32780}.
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分子量
- 61.9 kDa
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UniProt
- Q9DBA9
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途径
- DNA Damage Repair
抗原
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