STEAP2 Protein (AA 1-490) (Strep Tag)
-
- 抗原 See all STEAP2 蛋白
- STEAP2 (STEAP Family Member 2, Metalloreductase (STEAP2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-490
-
宿主
- 人
-
资源
- Cell-free protein synthesis (CFPS)
- 标记
- This STEAP2 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
-
MESISMMGSP KSLSETFLPN GINGIKDARK VTVGVIGSGD FAKSLTIRLI RCGYHVVIGS RNPKFASEFF PHVVDVTHHE DALTKTNIIF VAIHREHYTS LWDLRHLLVG KILIDVSNNM RINQYPESNA EYLASLFPDS LIVKGFNVVS AWALQLGPKD ASRQVYICSN NIQARQQVIE LARQLNFIPI DLGSLSSARE IENLPLRLFT LWRGPVVVAI SLATFFFLYS FVRDVIHPYA RNQQSDFYKI PIEIVNKTLP IVAITLLSLV YLAGLLAAAY QLYYGTKYRR FPPWLETWLQ CRKQLGLLSF FFAMVHVAYS LCLPMRRSER YLFLNMAYQQ VHANIENSWN EEEVWRIEMY ISFGIMSLGL LSLLAVTSIP SVSNALNWRE FSFIQSTLGY VALLISTFHV LIYGWKRAFE EEYYRFYTPP NFVLALVLPS IVILGKIILF LPCISRKLKR IKKGWEKSQF LEEGMGGTIP HVSPERVTVM
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
- 纯度
- approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- 质量等级
- custom-made
- Top Product
- Discover our top product STEAP2 蛋白
-
想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 HEK-293 CellsConjugateOrigin HumanPrice 90,901.44 ¥表达系统 Wheat germConjugate GST tagOrigin HumanPrice 6,623.29 ¥表达系统 Wheat germConjugate GST tagOrigin HumanPrice 6,623.29 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 6,177.32 ¥表达系统 HEK-293 CellsConjugate DYKDDDDK Tag,Strep TagOrigin HumanPrice 12,628.25 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
STEAP Family Member 2, Metalloreductase (STEAP2) (AA 1-490) protein (His tag)
custom-made STEAP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
STEAP Family Member 2, Metalloreductase (STEAP2) (AA 1-489) protein (His tag)custom-made STEAP2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
STEAP Family Member 2, Metalloreductase (STEAP2) proteinSTEAP2 宿主: 人 宿主: HEK-293 Cells Synthetic Nanodisc
STEAP Family Member 2, Metalloreductase (STEAP2) (AA 1-490) protein (GST tag)STEAP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
STEAP Family Member 2, Metalloreductase (STEAP2) (AA 2-100) protein (GST tag)STEAP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
-
The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. - 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
-
- 抗原
- STEAP2 (STEAP Family Member 2, Metalloreductase (STEAP2))
- 别名
- STEAP2 (STEAP2 产品)
- 背景
- Metalloreductase STEAP2 (EC 1.16.1.-) (Prostate cancer-associated protein 1) (Protein up-regulated in metastatic prostate cancer) (PUMPCn) (Six-transmembrane epithelial antigen of prostate 2) (SixTransMembrane protein of prostate 1),FUNCTION: Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane (By similarity). Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate (By similarity). Can also reduce Cu(2+) to Cu(1+) (By similarity). {ECO:0000250|UniProtKB:Q687X5, ECO:0000250|UniProtKB:Q8BWB6}.
- 分子量
- 56.1 kDa
- UniProt
- Q8NFT2
- 途径
- Transition Metal Ion Homeostasis
-
