电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

MAP10 Protein (AA 1-905) (Strep Tag)

custom-made MAP10 宿主: 人 宿主: Cell-free protein synthesis (CFPS) Recombinant > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
产品编号 ABIN3093696
发货至: 中国
  • 抗原 See all MAP10 products
    MAP10 (Microtubule-Associated Protein 10 (MAP10))
    蛋白类型
    Recombinant
    产品特性
    AA 1-905
    宿主
    • 2
    • 1
    资源
    • 2
    • 1
    Cell-free protein synthesis (CFPS)
    标记
    This MAP10 protein is labelled with Strep Tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    品牌
    AliCE®
    序列
    MAASLSERLF SLELLVDWVR LEARLLPSPA AAVEQEEEEE EKEQGEASSP RGLCPAVAFR LLDFPTLLVY PPDGPGAPAA EPWPGVIRFG RGKSCLFRLQ PATLHCRLLR TPLATLLLQL PPGRPTPTPQ LLGACDISLA TAAHRVVGPA ASGCSHRHRG RFPLHNRVGE RTGDIALAYR LTDLGSRLLS QLERPLTFTR TGGGAEVSPQ TQQERQQLQQ PASQPSPKEA DKPLGELEIP EAQKDLKEMV KSKAECDNVG SVENGKTNSV VTCSGAGNGR NVSSLNEEVT ELDMETNIFC PPPLYYTNLT QEKPPPAQAK ITIEPQMNAP EEMDDASPEK KRVNPPAHRS CLKHPSSAAH EHPPMLVNPP HIQNIGATNQ TCQTEQNRIN TIRQLPLLNA LLVELSLLYD QPVTSPAHIH PHLAWLYRTE DKKSPESSAK STCRSEAKKD KRSVGGCEKS VSLQYKKNQI ENYKEDKYSE KSSGALHKRV PKGRLLYGLT NTLRLRLKLT NPDMLVVHEK RELYRKRQSQ MLGTKFRIPS SKVKLLSSAE QSQKPQLPED KYLDSDASFT ENSDTSRQIS GVFDEPSTSK ETKLKYATEK KTVDCSKNRI NNVSLEEVVS PANSIIPERL TPTNILGGNV EMKIQSPCVF QQDAVVDRIV DKEIDIRQVK TTDNDILMAD ISDKRTGKNS CYENISELKY SDDLSSPCYS EDFCTSEDTS RSFKAHDSSS RTENPKHSQY TSKSSDTGVS KKKNSSDRSS ILSPPFSAGS PVHSYRKFHI SKTQDKSLEE ASSISASDLS STHWTEQKEN QIDQNSMHNS EITKRAQDIS VKTRSSWKSL EKSQSPQTSQ VSSYLPSNVS ELNVLDSSTS DHFEEGNDDV GSLNISKQCK DICELVINKL PGYTM
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    纯度
    > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    质量等级
    custom-made
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    12 months
  • 抗原
    MAP10 (Microtubule-Associated Protein 10 (MAP10))
    别名
    MAP10 (MAP10 产品)
    背景
    Microtubule-associated protein 10 (Microtubule regulator of 120 KDa),FUNCTION: Microtubule-associated protein (MAP) that plays a role in the regulation of cell division, promotes microtubule stability and participates in the organization of the spindle midzone and normal progress of cytokinesis. {ECO:0000269|PubMed:23264731}.
    分子量
    100.3 kDa
    UniProt
    Q9P2G4
You are here:
客服