FASTK Protein (AA 1-549) (Strep Tag)
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- 抗原 See all FASTK 蛋白
- FASTK (Fas-Activated serine/threonine Kinase (FASTK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-549
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宿主
- 人
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资源
- Cell-free protein synthesis (CFPS)
- 标记
- This FASTK protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 品牌
- AliCE®
- 序列
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MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS GLLLIPPVQP CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR WLGQNPSKVR AHHYSVALRR LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL EQERRLRLPP KPPPPLQPLL RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM VLLAQHLARH RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH ALIVRRYLSL LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS HKDIVAEGLR QLLGEEKYRQ DLTVPPGYCT DFLLCASSSG AVLPVRTQDP FLPYPPRSCP QGQAASSATT RDPAQRVVLV LRERWHFCRD GRVLLGSRAL RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL GLRWGPEGG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- 纯度
- approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- 质量等级
- custom-made
- Top Product
- Discover our top product FASTK 蛋白
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Fas-Activated serine/threonine Kinase (FASTK) (AA 1-511) protein (His tag)
custom-made FASTK 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fas-Activated serine/threonine Kinase (FASTK) (AA 1-549) protein (His tag)custom-made FASTK 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fas-Activated serine/threonine Kinase (FASTK) (AA 1-549) protein (GST tag)FASTK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fas-Activated serine/threonine Kinase (FASTK) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)FASTK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. - 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- FASTK (Fas-Activated serine/threonine Kinase (FASTK))
- 别名
- FASTK (FASTK 产品)
- 背景
- Fas-activated serine/threonine kinase (FAST kinase) (EC 2.7.11.1) (EC 2.7.11.8),FUNCTION: Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor. {ECO:0000269|PubMed:17135269, ECO:0000269|PubMed:7544399}., FUNCTION: [Isoform 4]: Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels. {ECO:0000269|PubMed:25704814}.
- 分子量
- 61.1 kDa
- UniProt
- Q14296
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