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Quick Overview for DLAT Protein (His tag) (ABIN3009179)
抗原
See all DLAT 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This DLAT protein is labelled with His tag.
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原理
- Mouse Dihydrolipoyl Transacetylase (DLAT) Protein
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序列
- TQV KGTGPEGRII KKDIDSFVPS KAAPAAAAAM APPGPRVAPA PAGVFTDIPI SNIRRVIAQR LMQSKQTIPH YYLSVDVNMG EVLLVRKELN KMLEGKGKIS VNDFIIKASA LACLKVPEAN SSWMDTVIRQ NHVVDVSVAV STPAGLITPI VFNAHIKGLE TIASDVVSLA SKAREGKLQP HEFQGGTFTI SNLGMFGIKN FSAIINPPQA CILAIGASED KLIPADNEKG FDVASVMSVT LSCDHRVVDG AVGAQW
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Biological Activity Comment
- Not tested
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Dihydrolipoyl Transacetylase (DLAT) (AA 169-273) protein (His-IF2DI Tag)
DLAT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Dihydrolipoyl Transacetylase (DLAT) (AA 1-647) protein (GST tag)DLAT 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Dihydrolipoyl Transacetylase (DLAT) (AA 93-640), (partial) protein (His tag)DLAT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Dihydrolipoyl Transacetylase (DLAT) protein (Myc-DYKDDDDK Tag)DLAT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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缓冲液
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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储存液
- Dithiothreitol (DTT), ProClin
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注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- DLAT (Dihydrolipoyl Transacetylase (DLAT))
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别名
- Dihydrolipoyl Transacetylase
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分子量
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Calculated MW: 29.0 kDa
Observed MW (SDS-PAGE): 29 kDa -
UniProt
- Q8BMF4
抗原
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