DLAT Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all DLAT 蛋白
- DLAT (Dihydrolipoyl Transacetylase (DLAT))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This DLAT protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human DLAT / DLTA protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DLAT 蛋白
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Dihydrolipoyl Transacetylase (DLAT) (AA 169-273) protein (His-IF2DI Tag)
DLAT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Dihydrolipoyl Transacetylase (DLAT) (AA 1-647) protein (GST tag)DLAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Dihydrolipoyl Transacetylase (DLAT) (AA 87-647) protein (His tag)Crystallography grade DLAT 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dihydrolipoyl Transacetylase (DLAT) (AA 86-642) protein (His tag)Crystallography grade DLAT 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Dihydrolipoyl Transacetylase (DLAT) (AA 93-640), (partial) protein (His tag)DLAT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Dihydrolipoyl Transacetylase (DLAT) proteinDLAT 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- DLAT (Dihydrolipoyl Transacetylase (DLAT))
- 别名
- Dlat,dlta (DLAT 产品)
- 别名
- DLAT Protein, Dmel\\CG5261 Protein, EP(2)0816 Protein, EP816 Protein, anon-WO0118547.121 Protein, DLTA Protein, PDC-E2 Protein, PDCE2 Protein, wu:fc14f10 Protein, wu:fc21f08 Protein, wu:fc86g11 Protein, wu:fj57d06 Protein, 6332404G05Rik Protein, midline uncoordinated Protein, dihydrolipoamide S-acetyltransferase L homeolog Protein, dihydrolipoamide S-acetyltransferase Protein, dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) Protein, muc Protein, dlat.L Protein, DLAT Protein, Dlat Protein, dlat Protein
- 背景
- This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95 % of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009].
- 分子量
- 59.6 kDa
- NCBI登录号
- NP_001922
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