NAD Synthetase 蛋白
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- 抗原
- NAD Synthetase
- 蛋白类型
- Recombinant
- 宿主
- Bacillus subtilis
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资源
- 大肠杆菌（E. Coli）
- 原理
- NAD Synthetase (B. subtilis) (rec.)
- 产品特性
- Full-length NAD synthetase (Bacillus subtilis).
- 纯度
- >98 % (SDS-PAGE)
- Biological Activity Comment
- ~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- 0.5 mg/mL (Lot specific)
- 浓度
- Lot specific
- 缓冲液
- In 50 mM TRIS-HCl, pH 8, containing 75 mM sodium chloride, 5 % glycerol and 5 mM DTT.
- 注意事项
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
- 储存条件
- -20 °C,-80 °C
- 储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
- 有效期
- 6 months
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- 抗原
- NAD Synthetase
- 背景
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NH(3)-dependent NAD(+) Synthetase, General Stress Protein 38, GSP38, Spore Outgrowth Factor B, EC6.3.1.5
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
- 分子量
- ~30.4kDa
- UniProt
- P08164
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