NAD Synthetase protein (His tag)
This Recombinant NAD Synthetase protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN6253505
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for NAD Synthetase protein (His tag) (ABIN6253505)
抗原
NAD Synthetase
蛋白类型
Recombinant
宿主
Mycobacterium tuberculosis
资源
大肠杆菌（E. Coli）
纯度
>98 % (SDS-PAGE)
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标记
- His tag
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原理
- NAD Synthetase (M. tuberculosis) (rec.) (His)
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特异性
- NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
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产品特性
- NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
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Biological Activity Comment
- ~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- 0.5 mg/mL (Lot specific)
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浓度
- Lot specific
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缓冲液
- In 20 mM TRIS-HCl, pH 7.8.
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注意事项
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
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储存条件
- -20 °C,-80 °C
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储存方法
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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有效期
- 6 months
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- NAD Synthetase
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背景
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NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
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分子量
- ~75.0kDa
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UniProt
- P0A5L6
抗原
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