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VEGFA Protein (Transcript Variant 6)

Name: VEGFA Protein (Transcript Variant 6)
SKU: ABIN2735217
Availability: OutOfStock

This Recombinant VEGFA protein is expressed in HEK-293 Cells.

产品编号 ABIN2735217

发货至: 中国

Datasheet as PDF

Quick Overview for VEGFA Protein (Transcript Variant 6) (ABIN2735217)

抗原

See all VEGFA 蛋白

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

蛋白类型

Recombinant

生物活性

Active

宿主

人

资源

HEK-293 Cells

应用范围

Antibody Production (AbP), Functional Studies (Func), Standard (STD), Protein Interaction (PI)

纯度

> 98 % , as determined by Coomassie stained SDS-PAGE.

Discover our top product VEGFA 蛋白

产品特性

Transcript Variant 6

特异性

Optimal preservation of protein structure, post-translational modifications and functions.

产品特性
- Recombinant human VEGF-A (transcript variant 6) protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
内毒素水平

Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment

The ED50 is 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.
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Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (Fc Tag)
VEGFA 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag,AVI tag,Biotin)
VEGFA 宿主: 小鼠宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active

Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) (Active) protein (His tag,AVI tag,Biotin)
VEGFA 宿主: 小鼠宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Vascular Endothelial Growth Factor A (VEGFA) protein
VEGFA 宿主: 大鼠宿主: 酵母菌 Recombinant > 95 % as determined by SDS-PAGE

Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA

Vascular Endothelial Growth Factor A (VEGFA) protein
VEGFA 宿主: 小鼠宿主: Yeast (Pichia pastoris) Recombinant > 95 % as determined by SDS-PAGE

Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) protein (GST tag)
VEGFA 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AP, AA

Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) protein (His tag)
VEGFA 宿主: 大鼠宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS

Vascular Endothelial Growth Factor A (VEGFA) protein
VEGFA 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS

应用备注

Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

限制

仅限研究用
浓度

> 50 μg/mL

缓冲液

5 mM citric acid, 5 mM NaHPO4, 0.15 M NaCl, pH 4.0

储存条件

-80 °C

储存方法

Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

别名

Vegf-A

背景

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

分子量

14 kDa

NCBI登录号

NP_001020541

途径

RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling