VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
蛋白类型
Recombinant
生物活性
Active
产品特性
Transcript Variant 2
宿主
小鼠
资源
大肠杆菌(E. Coli)
应用范围
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human VEGF-A (transcript variant 2) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 98 % , as determined by Coomassie stained SDS-PAGE.
内毒素水平
Less than 0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.
VEGFA
宿主: 小鼠
宿主: Yeast (Pichia pastoris)
Recombinant
> 95 % as determined by SDS-PAGE
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))