VEGFA Protein (Transcript Variant 2)
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北京 101111
Quick Overview for VEGFA Protein (Transcript Variant 2) (ABIN2735215)
抗原
See all VEGFA 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 2
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human VEGF-A (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Less than 0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 10^6 units/mg, determined by the dose dependent stimulation of HUVEC cells proliferation.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- > 50 μg/mL
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缓冲液
- 5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- Vegf-A
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分子量
- 14.1 kDa
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NCBI登录号
- NP_033531
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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