Splicing Factor 1 Protein (SF1) (Transcript Variant 3) (Myc-DYKDDDDK Tag)
SF1
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- 抗原 See all Splicing Factor 1 (SF1) 蛋白
- Splicing Factor 1 (SF1)
- 蛋白类型
- Recombinant
- 产品特性
- Transcript Variant 3
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Splicing Factor 1 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human Splicing factor 1 (SF1) (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SF1 蛋白
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Splicing Factor 1 (SF1) (AA 1-110) protein (GST tag)
SF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Splicing Factor 1 (SF1) (AA 1-548) protein (GST tag)SF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- Splicing Factor 1 (SF1)
- Abstract
- SF1 产品
- 背景
- This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3&apos splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
- 分子量
- 59.5 kDa
- NCBI登录号
- NP_973727
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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