Quick Overview for RSPO1 蛋白 (ABIN2731226)
抗原
See all RSPO1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human RSPO1 protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- R-spondin-1 enhances BMP-2 mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml.
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R-Spondin 1 (RSPO1) (AA 31-263) protein (His tag)
RSPO1 宿主: 人 宿主: CHO Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)RSPO1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (AA 21-146) protein (His tag)RSPO1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
R-Spondin 1 (RSPO1) (AA 21-146) protein (Fc Tag)RSPO1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)RSPO1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RSPO1 (R-Spondin 1 (RSPO1))
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别名
- Rspo1
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背景
- This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
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分子量
- 26.7 kDa
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NCBI登录号
- NP_001033722
抗原
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