PEG10 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for PEG10 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2728638)
抗原
See all PEG10 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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标记
- This PEG10 protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human PEG10 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Paternally Expressed 10 (PEG10) (AA 1-958) protein (His tag)
custom-made PEG10 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Paternally Expressed 10 (PEG10) (AA 1-708) protein (His tag)custom-made PEG10 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Paternally Expressed 10 (PEG10) (AA 1-325) protein (GST tag)PEG10 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PEG10 (Paternally Expressed 10 (PEG10))
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别名
- Peg10
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背景
- This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1 and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene.
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分子量
- 36.8 kDa
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NCBI登录号
- NP_001035242
抗原
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