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Quick Overview for PDGFA 蛋白 (ABIN2728536)
抗原
See all PDGFA 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human PDGFA protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is less than or equal to 1 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
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Platelet Derived Growth Factor A (PDGFA) (Active) protein
PDGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor A (PDGFA) (AA 1-211) protein (GST tag)PDGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet Derived Growth Factor A (PDGFA) (AA 94-194) (Active) protein (His tag)PDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Platelet Derived Growth Factor A (PDGFA) (AA 87-196) protein (His tag)PDGFA 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Platelet Derived Growth Factor A (PDGFA) proteinPDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Platelet Derived Growth Factor A (PDGFA) proteinPDGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Platelet Derived Growth Factor A (PDGFA) proteinPDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Platelet Derived Growth Factor A (PDGFA) proteinPDGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PDGFA (Platelet Derived Growth Factor A (PDGFA))
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别名
- Pdgfa
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分子量
- 25.5 kDa
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NCBI登录号
- NP_032834
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
抗原
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