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PDGFA Protein (Transcript Variant 2)

Name: PDGFA Protein (Transcript Variant 2)
SKU: ABIN2728534
Availability: OutOfStock

This Recombinant PDGFA protein is expressed in 大肠杆菌（E. Coli）.

Validation with Western Blot (PDGFA Protein (Transcript Variant 2))

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产品编号 ABIN2728534

发货至: 中国

Quick Overview for PDGFA Protein (Transcript Variant 2) (ABIN2728534)

抗原

See all PDGFA 蛋白

PDGFA (Platelet Derived Growth Factor A (PDGFA))

蛋白类型

Recombinant

生物活性

Active

宿主

人

资源

大肠杆菌（E. Coli）

应用范围

Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

纯度

> 95 % as determined by SDS-PAGE and Coomassie blue staining

Discover our top product PDGFA 蛋白

产品特性

Transcript Variant 2

特异性

Optimal preservation of protein structure, post-translational modifications and functions.

产品特性
- Recombinant human PDGFA (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
内毒素水平

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment

ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is less than or equal to 1 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
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Platelet Derived Growth Factor A (PDGFA) (Active) protein
PDGFA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active

Platelet Derived Growth Factor A (PDGFA) (AA 1-211) protein (GST tag)
PDGFA 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AP, AA

Platelet Derived Growth Factor A (PDGFA) (AA 94-194) (Active) protein (His tag)
PDGFA 宿主: 小鼠宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active

Platelet Derived Growth Factor A (PDGFA) protein
PDGFA 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS

Platelet Derived Growth Factor A (PDGFA) protein
PDGFA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS

Platelet Derived Growth Factor A (PDGFA) protein
PDGFA 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS

Platelet Derived Growth Factor A (PDGFA) protein
PDGFA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS

Platelet Derived Growth Factor A (PDGFA) (AA 87-196) protein (His tag)
PDGFA 宿主: 犬宿主: 酵母菌 Recombinant > 90 % as determined by Tris-Bis PAGE

Platelet Derived Growth Factor A (PDGFA) (AA 87-196) protein (His tag)
PDGFA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, Ct

Platelet Derived Growth Factor A (PDGFA) (AA 87-211) protein (His tag)
PDGFA 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC

应用备注

Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

限制

仅限研究用
缓冲液

Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

注意事项

Resuspend the protein in the desired concentration in proper buffer

储存条件

-80 °C

储存方法

Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原

PDGFA (Platelet Derived Growth Factor A (PDGFA))

别名

Pdgfa

背景

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

分子量

28.5 kDa

NCBI登录号

NP_002598

途径

RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling