Osteopontin Protein (Transcript Variant 2)
Quick Overview for Osteopontin Protein (Transcript Variant 2) (ABIN2728022)
抗原
See all Osteopontin (SPP1) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 2
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Osteopontin / SPP1 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
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Secreted phosphoprotein 1 (SPP1) (AA 17-300) protein (His tag)
SPP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 17-294) protein (His tag)SPP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Secreted phosphoprotein 1 (SPP1) proteinSPP1 宿主: 人 Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 宿主: 大鼠 宿主: HEK-293 Cells > 95 % AcA, CC Active
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- Osteopontin,spp1
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背景
- The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
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分子量
- 60 kDa
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NCBI登录号
- NP_000573
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途径
- Regulation of Cell Size
抗原
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