Nerve Growth Factor Protein (NGF)
This Recombinant Nerve Growth Factor protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for Nerve Growth Factor Protein (NGF) (ABIN2727244)
抗原
See all Nerve Growth Factor (NGF) 蛋白
Nerve Growth Factor (NGF)
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human NGF protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- determined by its ability to stimulate chick E9 DRG neurite outgrowth. ED50 is less than or equal to 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
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Nerve Growth Factor (NGF) (AA 122-239) protein (His tag)
NGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Nerve Growth Factor (NGF)
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别名
- Ngf
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背景
- This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene&aposs expression is associated with allergic rhinitis.
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分子量
- 13.5 kDa
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NCBI登录号
- NP_002497
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途径
- Regulation of Cell Size
抗原
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