Nerve Growth Factor Protein (NGF)
This Recombinant Nerve Growth Factor protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN7491868
发货至:
中国
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Quick Overview for Nerve Growth Factor Protein (NGF) (ABIN7491868)
抗原
See all Nerve Growth Factor (NGF) 蛋白
Nerve Growth Factor (NGF)
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
大肠杆菌（E. Coli）
应用范围
Cell Culture (CC), Cytotoxicity Test (CyTox)
纯度
≥98 % (HPLC)
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原理
- Recombinant mouse proNGF protein
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序列
- MEPYTDSNVP EGDSVPEAHW TKLQHSLDTA LRRARSAPTA PIAARVTGQT RNITVDPRLF KKRRLHSPRV LFSTQPPPTS SDTLDLDFQA HGTIPFNRTH RSKRSSTHPV FHMGEFSVCD SVSVWVGDKT TATDIKGKEV TVLAEVNINN SVFRQYFFET KCRASNPVES GCRGIDSKHW NSYCTTTHTF VKALTTDEKQ AAWRFIRIDT ACVCVLSRKA TRRG (the prodomain is shown in bold)
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR) and VPS10 domain-containing receptors (Sortilin and SorCS2)
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内毒素水平
- <0.1 EU per 1 μg of the protein by the LAL method.
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Biological Activity Comment
- proNGF is the preferred ligand of p75NTR 1. In contrast to its mature form proNGF induces apoptosis via p75NTR 1, but can also mediate cell survival in DRGs2.
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Nerve Growth Factor (NGF) (AA 122-239) protein
NGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Antigen Preadsorption Control: 0.4 - 4 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- Nerve Growth Factor (NGF)
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别名
- proNGF
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背景
- A recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
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分子量
- 50.4 kDa (dimer)
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途径
- Regulation of Cell Size
抗原
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