Midkine Protein (Transcript Variant 3)
Quick Overview for Midkine Protein (Transcript Variant 3) (ABIN2726002)
抗原
See all Midkine (MDK) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 3
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Midkine (transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10.0 ng/ml.
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) (Active) protein
MDK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-140) protein (His tag)MDK 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (His-Avi Tag)MDK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (His tag)MDK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-140) protein (His tag)MDK 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 1-143) protein (GST tag)MDK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (Fc Tag)MDK 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-143) protein (Fc Tag)MDK 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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别名
- Midkine
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背景
- This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
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分子量
- 13.4 kDa
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NCBI登录号
- NP_002382
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途径
- RTK signaling, M Phase, Skeletal Muscle Fiber Development
抗原
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