ASK1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for ASK1 Protein (Myc-DYKDDDDK Tag) (ABIN2725798)
抗原
See all ASK1 (MAP3K5) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This ASK1 protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human MEKK5 / ASK1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 115,504.49 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1-1380) protein (His tag)
custom-made MAP3K5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1-1374) protein (His tag)custom-made MAP3K5 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1180-1374) protein (His tag)MAP3K5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) proteinMAP3K5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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别名
- Mekk5,ask1
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背景
- Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells MAPKKK5 does not activate MAPK/ERK.
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分子量
- 154.4 kDa
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NCBI登录号
- NP_005914
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途径
- MAPK Pathway, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
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