IL-25 Protein (Transcript Variant 2)
Quick Overview for IL-25 Protein (Transcript Variant 2) (ABIN2723688)
抗原
See all IL-25 (IL25) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 2
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-25 / IL25 (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10-100ng.
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Interleukin 25 (IL25) (AA 33-177) protein (His tag)
IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (AA 33-177) protein (His-Avi Tag,Biotin)IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (Active) proteinIL25 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 25 (IL25) (AA 33-177) protein (Fc Tag)IL25 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 25 (IL25) (AA 17-169) protein (His tag)IL25 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 33-177) protein (His tag)IL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-177) protein (GST tag)IL25 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 25 (IL25) (Active) protein (Fc Tag)IL25 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Interleukin 25 (IL25) proteinIL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-25 (IL25) (Interleukin 25 (IL25))
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别名
- Interleukin-25,il25
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背景
- The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
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分子量
- 33.8 kDa
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NCBI登录号
- NP_758525
抗原
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