IL-15 Protein (Transcript Variant 3)
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北京 101111
Quick Overview for IL-15 Protein (Transcript Variant 3) (ABIN2723622)
抗原
See all IL-15 (IL15) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 3
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-15 / IL15 (transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Less than 0.01 ng per ug protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 0.10-0.50 ng/ml, corresponding to a specific activity of 2-10 x 10^6 units/mg, determined by the dose dependent stimulation of CTLL-2 cells proliferation.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- > 50 μg/mL
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缓冲液
- 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-15 (IL15) (Interleukin 15 (IL15))
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别名
- Interleukin-15,il15
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背景
- Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. [UniProtKB/Swiss-Prot Function]
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分子量
- 12.8 kDa
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NCBI登录号
- NP_000576
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途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
抗原
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