Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human Interleukin-15 / IL15 (transcript variant 3) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
内毒素水平
Less than 0.01 ng per ug protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.10-0.50 ng/ml, corresponding to a specific activity of 2-10 x 10^6 units/mg, determined by the dose dependent stimulation of CTLL-2 cells proliferation.
IL15
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
10 mM NaH2PO4, 150 mM NaCl, pH 7.2
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
IL-15 (IL15)
(Interleukin 15 (IL15))
别名
Interleukin-15,il15
背景
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. [UniProtKB/Swiss-Prot Function]