GAPDH Protein (Myc-DYKDDDDK Tag)
Quick Overview for GAPDH Protein (Myc-DYKDDDDK Tag) (ABIN2721688)
抗原
See all GAPDH 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This GAPDH protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human GAPDH protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-335) protein (His tag)
GAPDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-333) protein (His tag)GAPDH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) protein (GST tag)GAPDH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) protein (GST tag)GAPDH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) proteinGAPDH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- Gapdh
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背景
- This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
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分子量
- 35.9 kDa
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NCBI登录号
- NP_002037
抗原
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