Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human FGF acidic / FGF1 protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is less than or equal to 0.1 ng/ml, in the presence of 10 ug/ml heparin, corresponding to a specific activity of > 1 x 10^7 units/mg.
FGF1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.