FGF1 Protein (Transcript Variant 1)
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北京 101111
Quick Overview for FGF1 Protein (Transcript Variant 1) (ABIN2721038)
抗原
See all FGF1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human FGF acidic / FGF1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Assay #1: ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is less than or equal to 10 ng/ml, corresponding to a specific activity of > 1 x 10^5 units/mg. Assay #2: ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is less than or equal to 0.5 ng/ml in the presence of 10 ug/mL heparin, corresponding to a specific activity of > 2 x 10^6 units/mg.
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Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) (Active) protein (His tag)
FGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 1-155) (Active) protein (His tag,GST tag)FGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (Active) proteinFGF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) protein (His-IF2DI Tag)FGF1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 16-155) protein (His tag)FGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 46-155) protein (GST tag)FGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 1 (Acidic) (FGF1) (AA 1-155) protein (GST tag)FGF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 1 (Acidic) (FGF1) proteinFGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Fibroblast Growth Factor 1 (Acidic) (FGF1) proteinFGF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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别名
- Fgf Acidic,fgf1
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背景
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described.
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分子量
- 16 kDa
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NCBI登录号
- NP_000791
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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