CD3 Protein (CD3) (His tag)
Quick Overview for CD3 Protein (CD3) (His tag) (ABIN2713619)
抗原
See all CD3 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This CD3 protein is labelled with His tag.
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产品特性
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- Recombinant human CD3 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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CD3 (CD3) protein (Myc-DYKDDDDK Tag)
CD3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
CD3 (CD3) protein (DYKDDDDK-His Tag)CD3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CD3
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别名
- CD3
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背景
- The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
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分子量
- 12.79kD
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NCBI登录号
- NP_000724
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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