CD14 Protein (CD14) (Transcript Variant 1)
-
- 抗原 See all CD14 蛋白
- CD14
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
-
宿主
- 人
-
资源
- HEK-293 Cells
- 应用范围
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
-
- Recombinant human CD14 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by the dose dependent activation of NFkB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/uL to 200 ng/uL. The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/uL to 1.0 ng/uL bacterial LPS.
- Top Product
- Discover our top product CD14 蛋白
-
CD14 (CD14) (AA 16-345) protein (His tag)
CD14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 20-344) protein (His tag)CD14 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
CD14 (CD14) protein (His tag,Fc Tag)CD14 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
-
- 应用备注
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
-
- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- 抗原
- CD14
- 别名
- CD14 (CD14 产品)
- 别名
- CD14 molecule Protein, CD14 antigen Protein, CD14 Protein, Cd14 Protein
- 背景
- The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
- 分子量
- 36.4 kDa
- NCBI登录号
- NP_000582
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
-
