PYCARD Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for PYCARD Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2713165)
抗原
See all PYCARD 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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标记
- This PYCARD protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human CARD5 / PYCARD / ASC (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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PYD and CARD Domain Containing (PYCARD) (AA 1-195) protein (His tag)
custom-made PYCARD 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PYD and CARD Domain Containing (PYCARD) (AA 1-193) protein (His tag)custom-made PYCARD 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PYD and CARD Domain Containing (PYCARD) (AA 1-149) protein (GST tag)PYCARD 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PYCARD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PYCARD 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PYCARD (PYD and CARD Domain Containing (PYCARD))
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别名
- Card5,pycard,asc
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背景
- This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
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分子量
- 21.4 kDa
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NCBI登录号
- NP_037390
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途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
抗原
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