Quick Overview for CA5B 蛋白 (ABIN2713133)
抗原
See all CA5B 蛋白
CA5B
(Carbonic Anhydrase VB, Mitochondrial (CA5B))
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Antibody Production (AbP), Standard (STD)
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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产品特性
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- Recombinant human Carbonic anhydrase 5B protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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内毒素水平
- < 0.1 EU per μg protein as determined by LAL test
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Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 1-317) protein (GST tag)
CA5B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) protein (Myc-DYKDDDDK Tag)CA5B 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
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别名
- Carbonic Anhydrase 5b
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背景
- Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
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分子量
- 33.8 kDa
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NCBI登录号
- NP_009151
抗原
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