Caspase 3 Protein (CASP3)
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Quick Overview for Caspase 3 Protein (CASP3) (ABIN2688833)
抗原
See all Caspase 3 (CASP3) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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纯化方法
- Active caspase-3 was expressed in E.coli and purified. When expressed in E. coli, caspase-3 will spontaneously undergo autoprocessing to yield the subunits characteristic of the active enzyme. Cleavage of the Ac-DEVD-AMC substrate by recombinant human caspases-3, -6, -7, and -8. The activity of the caspases was analyzed by spectrofluorometry using an excitation at 380 nm and an emission wwavelength of 430-460 nm (peak is at 440 nm).
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Caspase 3 (CASP3) (AA 29-277) protein (His-IF2DI Tag)
CASP3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Caspase 3 (CASP3) (AA 1-277) protein (GST tag)CASP3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Caspase 3 (CASP3) (AA 176-277) proteinCASP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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BD Pharmingen™ Recombinant Human Active Caspase-3
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 50 mM Tris ( pH 8.0) with 100 mM NaCl and 50 mM imidazole
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储存条件
- 4 °C,-80 °C
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储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. The thawed active enzyme is generally stable at 4°C for about one week.
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- Caspase 3 (CASP3)
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别名
- Caspase-3
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原
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